Molecular biology, Cancer studies, Human genetics, Genetic markers, Biology, Cell biology, Human stem cells, Human cancer stem cells, Genomics, Gene expression, Medical and molecular laboratories skills, Molecular cloning techniques, Polymerase chain reaction (PCR) technique, Reverse transcription polymerase chain reaction (RT-PCR) technique, Real-time quantitative reverse transcription PCR (Real-Time qRT-PCR) technique, DNA extraction and purification, DNA sequencing, RNA isolation and purification, RNA sequencing, Proteins extraction, Western blotting, Tissue culture, Gene knockdown technique, Analysis of genes and proteins in normal and cancerous cells, Meta-analysis software, Analysis of DNA and RNA quality, Stem cell markers analysis, Extreme limitations dilution (ELDA) analysis, Cell viability assay and differentiation of human cells.
Using Image Lab Software, Quantity One 1-D Analysis Software, Geneious Software, ApE A plasmid Editor, SnapGene Software, NanoDrop Instrument, Quabit Fluorometer, CFX Manager Software, GNU Image Manipulation Program, Snagit Software, ExPASy Translate web tool, THE HUMAN PROTEIN ATLAS WEB, NEBcutter web tool, Ensembl Genome Browser, Bioinformatics Tools for Pairwise Sequence Alignment, Global Sequence Alignment Tool, science gateway Walter+Eliza Hall Bioinformatics, CancerMA Analysis.
Designing of PCR primers using the following web tools (i) Oligonucleotide Properties Calculator; (ii) Primer 3 Input; (iii) Primer 3 Plus.
I would like to offer special thanks to Dr. Ramsay McFarlane for all his guidance and advice. I would like to express my gratitude to Dr. David Pryce, Dr. Natalia Gomez Escobar and Dr. Jane Wakeman. I also appreciate all my colleagues at Bangor University, as I am deeply grateful for all their assistance and support.